MP has opened a new era in the field of organ transplantation and tissue medication.read more read lessĪbstract: Background Organ shortage is a growing problem, with a rising number of organs being harvested from extended criteria donors, and this trend will further continue to increase as organ donors are getting older and have more comorbidities. Moreover, experimental studies have investigated the role of MP in reducing ischemia reperfusion injury of lungs, kidneys, and livers by applying mesenchymal stem cells (MSCs), anti-inflammatory agents, cytotopic anti-coagulants, and defatting cocktails. MP has allowed longer organ preservation compared to conventional static cold storage and allowed the use of organs that might otherwise have been discarded. This article reviews the current status of MP with a focus on its role in organ pre-conditioning and therapeutic interventions prior to transplantation. Ex situ machine perfusion (MP) has emerged in an effort to expand the donor pool, by improving organ preservation, providing diagnostic information, and more recently, acting as a platform for organ improvement. Thus, our results indicate that oxygen supply is required for maintenance of the physiological function of biliary epithelial cells during liver transplant and suggest that a constant oxygen supply during liver isolation along with ex situ liver organ culture can enhance the repair of biliary epithelial cell injury during liver transplantation.read more read lessĪbstract: There is a vast discrepancy between the number of patients waiting for organ transplantation and the available donor organs. No significant change in cell morphology was observed in those cells between the two groups. We found that about 4.5% of the biliary epithelial cells in the experimental group proliferated compared with only 0.4% of cells in the control based on BrdU staining. After 6 hours of normothermic ex situ liver culture, morphology and DNA replication in hepatocytes and biliary epithelial cells were assessed and compared between the two groups. All livers were then connected to an ex situ liver culture system in the presence of bromodeoxyuridine (BrdU), a thymidine analogue and a marker for cell proliferation. Livers in the experimental group were isolated with a constant supply of oxygen. Livers in the control group were isolated following a standard procedure without oxygen supply. Twelve rat livers from DCD were divided into two groups. In this work, we have examined the effect of oxygen on proliferation of biliary epithelial cells in the rat livers obtained from donation after circulatory death (DCD). It has been a challenge to maintain the physiological function of biliary epithelial cells during liver transplantation. Among these complications, nonanastomotic biliary strictures (NAS) are especially common and they are frequently therapy resistant in part because biliary epithelial cells are more sensitive to warm ischemic injury than hepatocytes. CONCLUSIONS Our data demonstrate that the liver culture system established in this work can be used to culture whole livers ex situ in the absence of erythrocytes.read more read lessĪbstract: Biliary complications remain a major source of morbidity in liver transplant patients. The results from biochemical assays and histology analysis indicate that livers after the organ culture still maintain the full function. Metabolic biomarkers, bile and urea production, hepatic cell viability and histology analysis of biopsies were examined and newly proliferated hepatic cells labeled by BrdU were analyzed after 6 hours ex situ culture. Livers were isolated and connected to an Ex situ liver culture system using a standard culture medium RPMI1640 supplied with 10% of fetal bovine serum and sufficient dissolved oxygen under a normothermic condition for 6 hours. Twelve castrated male land race/farm young porcine livers were exposed to 30 min warm ischemia and 30 min cold perfusion. The aim of this work is to test whether cell culture condition in vitro could be used to culture whole livers ex situ without the use of erythrocytes. Shortage of donor liver and increased waiting time for liver transplantation necessitate the development of an organ culture system by which livers can be cultured and maintained ex situ for a prolonged period of time. Abstract: UNLABELLED Liver transplantation is an effective approach to end-stage liver disease.
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